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KMID : 0363620090300040093
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Journal of Korean Oriental Medicine
2009 Volume.30 No. 4 p.93 ~ p.107
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Effects of the Houttuyniae Herba Extract on the Membranous Nephropathy induced by Cationic Bovine Serum Albumin in Mice
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Jung Dae-Ho
Cho Chung-Sik
Kim Cheol-Jung
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Abstract
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Objective: Membranous nephropathy (MN) is one of the most common causes of nephrotic syndrome in adults. However, there is not a satisfactory treatment for MN. This study aimed to evaluate the effect of Houttuyniae Herba Extract (HHE) on MN induced by cationic bovine serum albumin (cBSA).
Methods: Mice were divided into 4 groups. The first group, Normal, was injected with saline. The second group, Control, was treated with cBSA (10mg/kg i.p) only. The third group, HHE-250, was treated with cBSA (10mg/kg i.p) and HHE (250mg/kg, p.o). The fourth group, HHE-500, was treated with cBSA (10mg/kg i.p) and HHE (500mg/kg, p.o). After treatment for 4 weeks, we measured change of body weight, 24 hrs proteinuria, serum albumin, total cholesterol, triglyceride, BUN, creatinine, IgA, IgM, IgG, TNF-, IL-1 levels and the mRNA expression of IFN-, IL-6, and IL-10. The morphologic changes of renal glomeruli were also observed with a light microscope and an electron microscope.
Results: The levels of 24 hrs proteinuria and serum triglyceride, BUN, IgG, TNF-, IL-1 significantly decreased in both HHE groups, while the level of serum albumin significantly increased in both HHE groups. The mRNA expression of IFN- and IL-6 in splenocytes considerably increased in both HHE groups. The mRNA expression of IL-10 in splenocytes considerably decreased in both HHE groups. In histological findings of kidney tissue, thickening of GBM decreased in both HHE groups.
Conclusions: This study shows that HHE might be effective for treatment of acute stage MN. More clinical data and studies are to be done for efficient application.
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KEYWORD
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Houttuyniae Herba extract (HHE), membranous nephropathy (MN), cationic bovine serum albumin (cBSA)
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